Coding

Part:BBa_K5013003:Design

Designed by: Zhang Yuxi   Group: iGEM23_Squirrel-CHN   (2023-10-11)


This is a phenylalanine degrading enzyme controlled by a hypoxic promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2346
    Illegal BamHI site found at 608
    Illegal XhoI site found at 659
    Illegal XhoI site found at 722
    Illegal XhoI site found at 740
    Illegal XhoI site found at 818
    Illegal XhoI site found at 1019
    Illegal XhoI site found at 1262
    Illegal XhoI site found at 2009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1178
    Illegal NgoMIV site found at 1424
    Illegal NgoMIV site found at 1592
    Illegal NgoMIV site found at 1735
    Illegal NgoMIV site found at 2069
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 719
    Illegal BsaI site found at 1049
    Illegal BsaI site found at 1055
    Illegal BsaI.rc site found at 2180
    Illegal SapI site found at 1009


Design Notes

no


Source

no

References